DOT Drug Test
A required test for controlled substances that CDL drivers must pass before employment, after accidents, and at random under 49 CFR Part 382.
DOT drug tests screen for 5 substance categories: marijuana metabolites, cocaine metabolites, opioids, amphetamines, and phencyclidine (PCP). Tests are conducted via urine sample at a certified collection site. The urinalysis in the DOT physical is separate — it checks for glucose and protein, not drugs. A positive result must be reported to the FMCSA Clearinghouse. The driver cannot return to safety-sensitive duties until completing the Return-to-Duty (RTD) process.
Last updated: May 28, 2026